Announcement
Starting on July 4, 2018 the Indonesian Publication Index (IPI) has been acquired by the Ministry of Research Technology and Higher Education (RISTEKDIKTI) called GARUDA Garba Rujukan Digital (http://garuda.ristekdikti.go.id)
For further information email to portalgaruda@gmail.com

Thank you
Logo IPI  
Journal > Microbiology Indonesia > In Vitro Modulation of Human Intestinal Microbiota by Mannoligosaccharides Synthesized from Amorphophallus muelleri Glucomannan

 

Full Text PDF (244 kb)
Microbiology Indonesia
Vol 7, No 4 (2013): November 2013
In Vitro Modulation of Human Intestinal Microbiota by Mannoligosaccharides Synthesized from Amorphophallus muelleri Glucomannan
DINOTO, ACHMAD ( Research Center for Biology, Indonesian Institute of Sciences (LIPI). Jalan Raya Jakarta-Bogor KM 46, Cibinong 16911, Jawa Barat, Indonesia)
WATUMLAWAR, CORY CORAZON ( Sekolah Tinggi Teknologi Industri dan Farmasi (STTIF), Jalan Kumbang 23, Bogor 16151, Jawa Barat, Indonesia)
YOPI, YOPI ( Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Jakarta-Bogor KM 46, Cibinong 16911,Jawa Barat, Indonesia)
Article Info   ABSTRACT
Published date:
30 Dec 2013
 
The corms of Amorphophallus muelleri Blume contain a large amount of glucomannan, a kind of polysaccharide that are commonly consumed by people as gelly foods. In order to improve the beneficial properties of glucomannan, we previously have established the enzymatic process to produce the mannoligosaccharides from flour of glucomannan using microbial mannanase. The effects of mannoligosaccharides on the growth modulation of human intestinal microbiota were investigated in this study. A set of in vitro single batch culture experiment was conducted to study the effect of mannooligosaccharides on human-origin Lactobacillus fermentum AA0014 and Lactobacillus plantarum FU0811. A modified MRS medium containing 10% (w/v) sucrose, glucomannan, and mannoligosaccharide was used instead of glucose as carbon source. The results showed the highest growth rate (0.13 h-1) with both L. fermentum AA0014 and L. plantarum FU0811 in the presence of mannooligosaccharides. We confirmed this result by a similar in vitro experiment using human fecal samples of six healthy adults as innocula and analyzed the microbial population by fluorescence in situ hybridization (FISH). Lactobacilli were proliferated higher in the presence of mannoligosaccharide than other carbon sources, yielding the microbial proportion as much of 10.9% of total microbiota. Overall, this study demonstrated the potential use of mannoligosaccharides synthesized from A. muelleri glucomannan as prebiotic candidate of modulating the beneficial human intestinal microbiota.
Copyrights © 2013