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Journal > Indonesian Journal of Chemistry > SECONDARY METABOLITE FROM ENDOPHYTIC FUNGI Aspergillus niger OF THE STEM BARK OF KANDIS GAJAH (Garcinia griffithii)

 

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Indonesian Journal of Chemistry
Vol 12, No 2 (2012)
SECONDARY METABOLITE FROM ENDOPHYTIC FUNGI Aspergillus niger OF THE STEM BARK OF KANDIS GAJAH (Garcinia griffithii)
Elfita, Elfita ( Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Jalan Raya Palembang Prabumulih Km 32, Indralaya, Ogan Ilir, South Sumatera, 30662)
Muharni, Muharni ( Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Jalan Raya Palembang Prabumulih Km 32, Indralaya, Ogan Ilir, South Sumatera, 30662)
Munawar, Munawar ( Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Jalan Raya Palembang Prabumulih Km 32, Indralaya, Ogan Ilir, South Sumatera, 30662)
Aryani, Septa ( Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Jalan Raya Palembang Prabumulih Km 32, Indralaya, Ogan Ilir, South Sumatera, 30662)
Article Info   ABSTRACT
Published date:
04 Jun 2012
 
Garcinia griffithii are known as kandis gajah including the Garcinia genus. This plant has been traditionally used by local communities Sarasah Bonta, Lembah Arau, West Sumatra, to treat various diseases including gout. Aspergillus niger was isolated from the tissues of the stem bark of Garcinia griffithii. The fungi strain was identified base on colony and cell morphology characteristic. Aspergillus niger cultured in media 5L Potatos Dextose Broth (PDB) for 8 weeks and filtered. Media that already contains secondary metabolites are partitioned using ethyl acetate solvent in 5 L (twice), followed by evaporation. Furthermore, the extract is separated by chromatographic techniques to obtain a pure compound of white crystal. The molecular structures of isolated compounds are determined by spectroscopic methods including IR, 1H-NMR, 13C-NMR, HMQC, HMBC, and COSY. The compound was determined as phenolic (1).
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