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Journal > Media Kedokteran Hewan > Induksi Apoptosis Senyawa Andrografolida dari Sambiloto (Andrographis paniculata Nees) terhadap Kultur Sel Kanker

 

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Media Kedokteran Hewan
Vol 21, No 3 (2005): Media Kedokteran Hewan
Induksi Apoptosis Senyawa Andrografolida dari Sambiloto (Andrographis paniculata Nees) terhadap Kultur Sel Kanker
Article Info   ABSTRACT
Published date:
14 Jun 2014
 
The objective of this research was to evaluate the activity of andrographolide extracted from sambiloto (Andrographis paniculata Nees)   in induction of apoptosis in HeLa cells culture in vitro. This research consisted of two phases, the first phase was isolation and identification of andrographolide. The HeLa cells were incubated in RPMI media and Fetal Bovine Serum 10%. The concentration of   andrographolide  were  0 ;  0.05 ; 0.10 ; 0.25 ; 0.50 ; 1.00 ; 2.50 ; 5 ; 10 ; 25 ; 50 ; 100 ; 250 µg/ml dissolved in DMSO. Each andrographolide concentrations was added into HeLa cells culture and then incubated for 24 hours at 37oC in CO2 incubator. Apoptosis induction was determined by ethidium bromide-acridine orange exclusion and analyzed by flourecent microscope. The result of this research showed the andrografolide extracted from sambiloto (Andrographis paniculata Nees) induced apoptosis activity in HeLa cells culture in vitro with IC50 of 109.90µg/ml. The result of this experiment suggested to study the effect of andrografolide on expression of gene responsible for apoptosis mechanism in cancer cells in vitro. Key words: Andrographis paniculata Nees, andrographolide, apoptosis
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