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Journal > Jurnal Ilmu-Ilmu Peternakan > Antibody against progesterone in local rabbit following low dose of progesterone injection

 

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Jurnal Ilmu-Ilmu Peternakan
Vol 21, No 1 (2011)
Antibody against progesterone in local rabbit following low dose of progesterone injection
Suyadi, Suyadi ( Fakultas Peternakan Universitas Brawijaya)
Wahjuningsih, Sri ( Department of Animal Production, Faculty of Animal Husbandry, Brawijaya University)
Rachmawati, Achadiah ( Department of Animal Production, Faculty of Animal Husbandry, Brawijaya University)
Srianto, P. ( Faculty of Veterinary Medicine, University of Airlangga, Surabaya Indonesia)
Pangestu, M. ( Faculty of Veterinary Medicine, University of Airlangga, Surabaya Indonesia)
Article Info   ABSTRACT
Published date:
01 Apr 2011
 
ABSTRACT: Antibody against progesgerone was produced from serum of local rabbit following low dose of progesterone injection: While a control group (Control; n=5) was injected with Freunds adjuvant solution in aquadest, the treatment groups were either firstly injected with progesterone conjugated to Freunds Adjuvant (P--CFA, 150 p.l : 150 pl) or progesterone conjugated to Freunds Adjuvant and bovine serum albumin (P-CFA-BSA; 135 p;l : 150 tt1 : 15 gl). Twice boostering injections were adminstered using incomplete Freunds Adjuvant on day 14 and 52 after first immunization. Weekly bleeding for serum collection were done from 1 week following first booster immunization to week 10, Using ELISA technique it was shown that the antibody titer to progesterone after first and second booster immunization in the P-CFA groug was higher than Control- and P-CFA-BSA groups. The antibody titer in the P-CFA-BSA remained low similar in the Control group:Key words: antibody; progesterone; rabbit
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