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Journal > MARINA CHIMICA ACTA > KONVERSI KHITIN DARI KULIT KEPITING (Scylla serrata) MENJADI KHITOSAN DENGAN ENZIM KHITIN DEASETILASE

 

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MARINA CHIMICA ACTA
Vol 5, No 1
KONVERSI KHITIN DARI KULIT KEPITING (Scylla serrata) MENJADI KHITOSAN DENGAN ENZIM KHITIN DEASETILASE
Article Info   ABSTRACT
Published date:
24 Apr 2012
 
Chitin from crab shell (Scylla serrata) can be converted into chitosan using the enzyme of chitin deacetylase isolated from thermophillic microbe. Conversion process  initiated with microbe rejuvenation, grown in solid medium at temperature 55 oC and pH 7,0. Next optimum time determination producing the enzyme, fractionation of enzyme and producing the chitosan and finally analysed Infra Red spectrophotometry. Thermophillic microbe can yield the enzyme with optimum activity in  three to four days and fractionated with the amonium sulfate 40 % (w/v). Infra red spectrosgram data, chitosan was obtained with the deacetylated degree between 66.53 – 78.9 %, water content 5.75 – 7.56 % and ash content between 33.26 – 34.25 %.   Keywords :  Thermofilik microbe,  crab shell, chitin and  chitin deacetylase
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