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Journal > MARINA CHIMICA ACTA > USAHA BIOKONVERSI KITIN ASAL KEPITING RAJUNGAN MENJADI KHITOSAN

 

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MARINA CHIMICA ACTA
Vol 4
USAHA BIOKONVERSI KITIN ASAL KEPITING RAJUNGAN MENJADI KHITOSAN
Article Info   ABSTRACT
Published date:
23 Apr 2012
 
Enzymatic bioconversion of chitosan from crab shell chitin using thermophillic bacterium has been carried out. With chitinolytic optimum activity, called M-1 isolator, at 550C and pH 7.0, the microbe could grow best at fourth day of fermentation with O.D. of 0.62 as well as the enzyme at 7.03 UA/mL. With the same optimum condition, deacetylated chitin enzyme, utilised for converting chitin into chitosan, was also produced at 7.95 UA/mL. After further purification with ammonium sulfate 40%, chitosan was produced, examined, and confirmed accordingly. Keywords : Bioconversion, chitin, shell, chitosan, fermentation
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