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Journal > JURNAL MIPA UNSRAT ONLINE > Isolasi dan Karakterisasi Barcode DNA Tanaman Nusa indah putih (Mussaenda frondosa) Berdasarkan Gen matK

 

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JURNAL MIPA UNSRAT ONLINE
Vol 4, No 2 (2015)
Isolasi dan Karakterisasi Barcode DNA Tanaman Nusa indah putih (Mussaenda frondosa) Berdasarkan Gen matK
Article Info   ABSTRACT
Published date:
06 May 2015
 
Telah dilakukan penelitian untuk menentukan urutan nukleotida dari barcode DNA tanaman Nusa indah putih berdasarkan gen matK dan mengkarakterisasi matK tanaman nusa indah putih dengan analisis in-silico. Isolasi DNA dari tanaman Nusa indah putih telah dilakukan berdasarkan manual prosedur dari InnuPrep Plant DNA kit yang dilanjutkan dengan amplifikasi dengan metode Polymerase Chain Reaction (PCR) menggunakan primer forward matK-1RKIM-f dan primer reverse matK-3FKIM-r kemudian dielektroforesis dan disekuensing. Sekuensing tanaman  Nusa  indah  putih  menghasilkan  kromatogram  yang  berkualitas  tinggi, dengan panjang sekuens 843 bp. Hasil Analisis in-silico menunjukan matK tanaman nusa indah putih bersifat basa, stabil, dan berinteraksi baik dengan air.A study to determine the nucleotide sequence of the DNA barcode Nusa indah putih plants based on gene matK and to characterize the matK of Nusa indah putih plant using in-silico analysis had been done. Isolation of DNA from Nusa indah putih plant was conducted by manual procedures of InnuPrep Plant DNA kit, followed by amplification using Polymerase Chain Reaction (PCR) method using matK-1RKIM-f as forward primer and matK-3FKIM-r as reverse primer then electrophoresis and sequenced. Nusa indah putih plants sequencing produced a high-quality chromatogram produce, with a length of 843 bp sequences. Results of in-silico analysis showed that matK Nusa indah putih plant is a base, stable, and well-interacted with water.
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