Announcement
Starting on July 4, 2018 the Indonesian Publication Index (IPI) has been acquired by the Ministry of Research Technology and Higher Education (RISTEKDIKTI) called GARUDA Garba Rujukan Digital (http://garuda.ristekdikti.go.id)
For further information email to portalgaruda@gmail.com

Thank you
Logo IPI  
Journal > HAYATI Journal of Biosciences > Characterization of Bacteriophage Specific to Bacillus pumilus from Ciapus River in Bogor, West Java, Indonesia

 

Full Text PDF (2,344 kb)
HAYATI Journal of Biosciences
Vol 22, No 1 (2015): January 2015
Characterization of Bacteriophage Specific to Bacillus pumilus from Ciapus River in Bogor, West Java, Indonesia
KUSMIATUN, ANIK ( Department of Biology, Faculty of Mathemathics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680)
RUSMANA, IMAN ( Department of Biology, Faculty of Mathemathics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680)
BUDIARTI, SRI ( Department of Biology, Faculty of Mathemathics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680)
Article Info   ABSTRACT
Published date:
02 Jan 2015
 
Bacillus pumilus is a spore-forming bacteria that is rod-shaped, gram positive, and aerobic. B. pumilus produced pumilacidins, known to have toxic effects on epithelial cells. Antibiotics were usually used to treat the disease caused by bacteria. Antibiotic typing test of B. pumilus indigenous from sewage water showed that this isolate was resistant to ampicillin and clindamycin. An alternative way was by application of bacteriophages as biocontrol agents to reduce B. pumilus in environment. The aim of this study were to isolate and characterize B. pumilus bacteriophage isolated from Ciapus River in Bogor, West Java. Bacteriophages infecting B. pumilus were isolated from river water using the double agar overlay method. Phages were defined by plaque morphology, structure, host range, and characteristic of molecular weight protein phage. Phage FBa1, FBa2, and FBa3 had narrow host range and they were specific for infecting B. pumilus. Electron microscope observation showed that phage FBa1 had icosahedral head without tail (166.67 nm in diameter), so it is called phage-like particles. Characterization of phage FBa1 by SDS-PAGE showed five proteins band. Molecular weight of FBa1 proteins was 70.9, 54.9, 33.8, 28.3, and 21.4 kDa.
Copyrights © 2015