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Journal > Vektora > EFIKASI Bacillus thuringiensis H-14 ISOLAT SALATIGA SEDIAAN BUBUK DAN CAIR TERHADAP JENTIK Culex quinquefasciatus

 

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Vektora
Vol 7, No 2 OKT (2015)
EFIKASI Bacillus thuringiensis H-14 ISOLAT SALATIGA SEDIAAN BUBUK DAN CAIR TERHADAP JENTIK Culex quinquefasciatus
Anggraeni, Yusnita M. ( Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Salatiga)
Rahardianingtyas, Esti
Wianto, Rendro
Article Info   ABSTRACT
Published date:
10 Oct 2015
 
AbstrakFilariasis merupakan salah satu penyakit tular vektor yang masih menjadi masalah di Indonesia dengan prevalen­si­19%­pada­tahun­2009.­Salah­satu­nyamuk­yang­menjadi­vektor­filariasis­adalah­Culex­quinquefasciatus.Pengendalian nyamuk vektor dengan Bacillus thuringiensis H­14 (Bt H­14) isolat Salatiga sediaan cair dan bubuk­merupakan­salah­satu­cara­mengendalikan­filariasis­secara­hayati.­Penelitian­ini­bertujuan­untukmenentukan­efikasi­Bt­H-14­isolat­Salatiga­sediaan­cair­dan­bubuk­terhadap­Cx­quinquefasciatus.­Penelitiandilakukan dengan membuat Bt H­14 isolat Salatiga sediaan cair dan bubuk, menghitung jumlah sel dan spora pada masing­masing sediaan serta mengujikan pada jentik Cx. quinquefasciatus hasil pemeliharaan insektarium B2P2VRP Salatiga. Nilai kematian kemudian dianalisis menggunakan analisis Probit Hasil penelitian menunjukkan sediaan cair memiliki jumlah sel 1,55x108 sel/ml sedangkan sediaan bubuk 1,72x106 sel/g. Nilai LC90 Bt H­14 sediaan cair dan bubuk terhadap Cx. quinquefasciatus berturut­turut 0,056 ppm dan 10,94 ppm. Hal ini menunjukkan potensi keduanya dalam membunuh jentik Cx. quinquefasciatus sebagai vektor­filariasis.Kata kunci:­Bacillus­thuringiensis,­cair,­bubuk,­efikasi,­Culex­quinquefasciatus,AbstractFilariasis as a vector­borne disease become a problem in Indonesia with prevalence of 19% in 2009. Culex quinquefasciatus is one of its vectors. Larvae control using Salatiga isolate of Bacillus thuringiensis H­14 liquid and­powder­preparations­are­filariasis­mosquito­control­method.­This­study­aimed­to­determine­the­efficacyof Bt H­14 isolates Salatiga liquid and powder preparations against Cx. quinquefasciatus. The study was conducted by formulating Salatiga isolate Bt H­14 liquid and powder preparation, cells and spores counting of Bt H­14 in each preparation, and conducting the toxicity assay on Cx. quinquefasciatus larvae. The mortality was analyzed using Probit analysis. The results showed that liquid preparations had 1,55x108 cells/ml while the powder preparation had 1,72x106 cells/g. LC90 value of Bt H­14 liquid and powder preparations against Cx. quinquefasciatus were respectively 0.056 ppm and 10.94 ppm. This shows the potential of both preparations in killing Cx. quinquefasciatus­larvae­as­vectors­of­filariasis.Keywords: Bacillus thuringiensis,­liquid,­powder,­efficacy,­Culex quinquefasciatus
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