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Journal > INDONESIAN JOURNAL OF PHARMACY > Isolation of Tracheopasmolytic Compounds From Piper cubeba Fruits

 

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INDONESIAN JOURNAL OF PHARMACY
Vol 14 No 3, 2003
Isolation of Tracheopasmolytic Compounds From Piper cubeba Fruits
., Wahyono ( Faculty of Pharmacy, Gadjah Mada University, Yogyakarta)
Hakim, L. ( Faculty of Pharmacy, Gadjah Mada University, Yogyakarta)
Wahyuono, S. ( Faculty of Pharmacy, Gadjah Mada University, Yogyakarta)
Mursyidi, A. ( Faculty of Pharmacy, Gadjah Mada University, Yogyakarta)
Verpoorte, R. ( Division of Pharmacognosy LACDR, Gorleus Laboratories, Leiden University)
Timmerman, H. ( Division of Medicinal Chemistry, Department of Pharmacochemistry, Vrije Universiteit)
Article Info   ABSTRACT
Published date:
01 Jul 2005
 
The fruits of Piper cubeba (kemukus;Javanese)have been used in traditional medicine to treat illnesses such as asthma. Extracts were prepared by gradual maceration of the dried fruits with n-hexane, and by extraction of the residue with ethanol, and were tested for their tracheospasmolytic effects on isolated guinea-pig trachea contracted with metacholine at a dose of 0.25 mg/ml. Both extracts demonstrated spasm inhibition but the effect of the ethanol extract was lower than that of the n-hexane extract. However, TLC analysis did not distinguish between the extracts. The ethanol extract was then partitioned with n-hexane, and the first and second n-hexane extracts were combined and dried before further fractionation using vacuum liquid chromatography. The fractions were tested for tracheospasmolytic effects. Two active fractions were obtained and separated by column chromatography and preparative TLC. Four pure compounds were obtained. One of these is a lignan compound and identified as dihydrocubebin, had tracheospasmolytic activities.Key words: Piper cubeba; lignan, dihydrocubebin; tracheospasmolytic effect.
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