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Journal > Malang Neurology Journal > UCAO (UNILATERAL CEREBRAL ARTERY OCCLUSSION) METHOD INCREASES THE LEVEL OF MMP- 9 BRAIN TISSUE IN RATS MODEL OF ISCHEMIC STROKE

 

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Malang Neurology Journal
Vol 2, No 2 (2016)
UCAO (UNILATERAL CEREBRAL ARTERY OCCLUSSION) METHOD INCREASES THE LEVEL OF MMP- 9 BRAIN TISSUE IN RATS MODEL OF ISCHEMIC STROKE
Article Info   ABSTRACT
Published date:
27 Sep 2016
 
Background. For the last 5 years, 15.4% of total population died because of stroke, which 42.9% of those are caused by ischemic stroke. UCAO (Unilateral Cerebral Artery Occlusion) is a stroke induction method by ligating mice’s carotid artery for 45 minutes. Thus, giving a hypoxic condition similar to stroke attack in human. This method is less complicated and far more efficient. MMP-9 is a stroke marker which is assayed by ELISA from the blood of test animal.Objective. This research was conducted to prove UCAO (Unilateral Cerebral Artery Occlusion) method is capable to raise MMP-9 concentration in mice’s blood.Methods. This research was an experimental laboratory research with post-test only controlled group design. 8 male rats (8-10 weeks) were divided into 2 groups, control and treatment which would be inducted into stroke by UCAO method. A day after the treatment group had been induced to stroke, both group were tested to measure the MMP-9 blood concentration through ELISA.Results. In this research, UCAO method had increased MMP-9 blood concentration in treatment group, compared to the control group. It is proved by the statistic tests, Mann-Whitney and Kruskal-Wallis, which showed a significant increase in treatment group (p < 0.05).Conclusion. Based on this result, it can be concluded that UCAO method is accepted as a method to create an ischemic stroke mice model.
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