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Journal > Pharmaceutical Sciences and Research (PSR) > PURIFIKASI INHIBITOR ATPASE/RNA HELIKASE VIRUS JAPANESE ENCEPHALITIS DARI STREPTOMYCES CHARTREUSIS

 

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Pharmaceutical Sciences and Research (PSR)
Vol 6, No 2 (2009)
PURIFIKASI INHIBITOR ATPASE/RNA HELIKASE VIRUS JAPANESE ENCEPHALITIS DARI STREPTOMYCES CHARTREUSIS
Elfita, Lina ( Program studi Farmasi Fakultas Kedokteran dan Ilmu Kesehatan Universitas Islam Syarif Hidayatullah)
Ratnakomala, Shanti ( Pusat Penelitian Bioteknologi-LIP)
Suryadi, Herman ( Departemen Farmasi FMIPA UI)
Lisdiyanti, Puspita ( Pusat Penelitian Bioteknologi-LIPI)
Utama, Andi ( Pusat Penelitian Bioteknologi-LIPI)
Article Info   ABSTRACT
Published date:
14 Aug 2012
 
Japanese encephalitis virus (JEV) is a neuropathogenic virus commonly caused cen-tral nervous diseases such as meningitis and severe encephalitis. Although vaccine has been developed, no specific and effective drug is available so far. We previouslycarried out a screening of inhibitor of JEV RNA helicase, an enzyme that essential for virus replication, from Actinomycetes and found that Streptomyces chartreusis produce the inhibitor of JEV RNA helicase. In this study, an extracellular proteinwhich has inhibition activity on ATPase activity of JEV RNA helicase was purified from supernatant of Streptomyces chartreusis culture by ammonium sulfate pre-cipitation and size exclusion chromatography. SDS-PAGE analysis showed a singleband with aproximate molecular mass of 11,4 kDa, suggesting that the inhibitor was successfully purified into a single protein.Key words : Japanese encephaliti virus, RNA helikase Inhibitor, ATPase, Strepto-myces chartreusis.
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