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Journal > Jurnal Teknologi Dan Industri Pangan > IDENTIFIKASI ANTOSIANIN BUAH DUWET (Syzygium cumini) MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI - DIODE ARRAY DETECTION [Identification of Anthocyanins in Jambolan Fruit (Syzygium cumini) by High Performance Liquid Chromatography - Diode Array Detecti

 

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Jurnal Teknologi Dan Industri Pangan
Vol 20, No 2 (2009): Jurnal Teknologi dan Industri Pangan
IDENTIFIKASI ANTOSIANIN BUAH DUWET (Syzygium cumini) MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI - DIODE ARRAY DETECTION [Identification of Anthocyanins in Jambolan Fruit (Syzygium cumini) by High Performance Liquid Chromatography - Diode Array Detecti
Article Info   ABSTRACT
Published date:
16 Dec 2010
 
IDENTIFIKASI ANTOSIANIN BUAH DUWET (Syzygium cumini) MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI - DIODE ARRAY DETECTION [Identification of Anthocyanins in Jambolan Fruit (Syzygium cumini) by High Performance Liquid Chromatography - Diode Array Detection] Puspita Sari1)*, Christofora Hanny Wijaya2), Dondin Sajuthi3), dan Unang Supratman4) 1) Jurusan Teknologi Hasil Pertanian,Fakultas Teknologi Pertanian, Universitas Jember  Kampus Tegalboto Jl. Kalimantan I, Jember 68121 2) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor Kampus IPB Darmaga, Bogor 16680 3) Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680  4) Jurusan Kimia, FMIPA, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Jatinangor, Sumedang 45363   Diterima  1 Juli  2009 / Disetujui  7 Desember 2009   ABSTRACT   The aim of this study was to determine the content of total monomeric anthocyanins and to identify the anthocyanin composition of jambolan fruit (Syzygium cumini) growing in Indonesia. Anthocyanins were extracted with methanol containing 0.1% HCl, removed from non-polar impurities by partition with chloroform and purified in a C18 solid phase cartridge. Anthocyanins were identified by high-performance liquid chromatography with diode array detection (HPLC-DAD), UV-visible spectral analysis, and physico-chemical reactions. Using pH-differential method, it was known that the content of total monomeric anthocyanin in the ripe fruit was 161 mg/100 g fresh fruit (731 mg/100 g skin on wet basis or 3430 mg/100 g skin on dry basis), based on cyanidin-3-glucoside. Furthermore, five anthocyanins without acylating groups were identified as 3,5-diglucoside derivatives of delphinidin (41.29%), petunidin (27.79%), malvidin (25.60%), cyanidin (4.19%), and peonidin (1.13%). These results demonstrate that the skin of jambolan fruit especially the ripe one is rich in anthocyanin, with wide range in anthocyanidin backbone (five aglycons).   Key words: jambolan fruit, Syzygium cumini, anthocyanin, HPLC-DAD
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