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Journal > Agroscientiae > PERBANYAKAN IN VITRO PADA TANAMAN JERUJU (Hydrolea spinosa L.) DENGAN BERBAGAI KONSENTRASI ZAT PENGATUR TUMBUH

 

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Agroscientiae
Vol 19, No 1 (2012)
PERBANYAKAN IN VITRO PADA TANAMAN JERUJU (Hydrolea spinosa L.) DENGAN BERBAGAI KONSENTRASI ZAT PENGATUR TUMBUH
Article Info   ABSTRACT
Published date:
10 Apr 2013
 
Hydrolea spinosa(jeruju) is one of the potential plant for antimalarial medicine according to local people in Kayu Rabah Village in South Kalimantan. The growth of these auxilarry shoots of this plant will be inhibited when is grown on the soil medium due to the different environment of original habitat (swamp areal). Therefore, tissue culture is an alternative propagation of this plant. The objectives of this research were to study in vitro micropropagation in H. spinosa. Shoot induction and proliferation was studied using shoot tip and node segments in MS medium with various concentration of BAP (0.0, 0.5, 1.0, 2.0, 3.0, 4.0, and 5.0 mg L-1 ). Shoot elongation was done using different strength of MS salts (full-, a half-, one-quarter strength) and with or without addition of 1.0 mg L-1 gibberellin (GA3). The result showed that cytokinin level produced a significant response on the numbers of shoot per explants and also showed effect on node number. Both of shoot tip and node segments had a similar potential as explants in shoot induction and proliferation. The half strength of MS salts produced root with vigorous planlets. The planlets produced from elongation phase were acclimatized and had survival rate up to 75%.
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