Announcement
Starting on July 4, 2018 the Indonesian Publication Index (IPI) has been acquired by the Ministry of Research Technology and Higher Education (RISTEKDIKTI) called GARUDA Garba Rujukan Digital (http://garuda.ristekdikti.go.id)
For further information email to portalgaruda@gmail.com

Thank you
Logo IPI  
Journal > Jurnal Agritech Fakultas Teknologi Pertanian UGM > A SIMPLE AND SENSITIVE METHOD FOR DETERMINATION OF SUGAR CONTENT IN FRUITS AND CULTURE MEDIA DURING FERMENTATION

 

Full Text PDF (42 kb)
Jurnal Agritech Fakultas Teknologi Pertanian UGM
Vol 29, No 04 (2009)
A SIMPLE AND SENSITIVE METHOD FOR DETERMINATION OF SUGAR CONTENT IN FRUITS AND CULTURE MEDIA DURING FERMENTATION
Article Info   ABSTRACT
Published date:
24 Mar 2012
 
A colorimetric for sugar determination using cuprammonium reagent containing strong base has been improved with a heating process after a reaction started.  The reaction was started after the sugar or sample containing sugar was added to the cuprammonium reagent consisted of 1 mM CuSO – 0,4 M (NH ) SO – 0,6 M NaOH).  This heat­ ing process could enhance the formation of sugar­cupper ammonium complex, so that the intensity of absorbance increased at wavelength of 280 nm. This simple and sensitive method was applicable for various sugars detection such as sugar fraction after HPLC separation, sugar content in biological extract or in culture medium during fermentation. Interestingly, different from previous report, this method was specific for non polyol sugars.
Copyrights © 2012