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Journal > Jurnal Kedokteran Maranatha > Analisis DNA Bacillus sp. BAC4 Hasil Polymerase Chain Reaction (PCR) dengan Primer-Primer yang Dirancang dari Gen pga

 

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Jurnal Kedokteran Maranatha
Vol 8, No 1 (2008)
Analisis DNA Bacillus sp. BAC4 Hasil Polymerase Chain Reaction (PCR) dengan Primer-Primer yang Dirancang dari Gen pga
Onggowidjaja, Philips ( Universitas Kristen Maranatha)
Moeis, Maelita Ramdani ( Institut Teknologi Bandung)
Ratnaningsih, Enny ( Institut Teknologi Bandung)
Article Info   ABSTRACT
Published date:
08 Dec 2010
 
Bacillus sp. BAC4 DNA was amplified using PCR method employing primers designed from pga genes of 5 bacteria (Arthrobacter viscosus, Bacillus megaterium, Escherichia coli, Kluyvera citrophila, and Providentia rettgeri). Analysis  of DNA base direct sequencing of PCR products showed the highest homology of Bacillus sp. BAC4 DNA with Bacillus subtilis DNA at the region from citG to yirG.  This fact states that primers designed from pga genes of 5 bacteria has not been successful in amplifying Bacillus sp. BAC4 pga gene, and that Bacillus sp.  BAC4 pga gene is predicted to be quite different from the existing data of pga genes available at Genbank.  Analysis of DNA base direct sequencing amplified using the pga primers shows the highest homology to the DNA of Bacillus subtilis.
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