Announcement
Starting on July 4, 2018 the Indonesian Publication Index (IPI) has been acquired by the Ministry of Research Technology and Higher Education (RISTEKDIKTI) called GARUDA Garba Rujukan Digital (http://garuda.ristekdikti.go.id)
For further information email to portalgaruda@gmail.com

Thank you
Logo IPI  
Journal > Chem Info > PEMURNIAN SELULASE DARI ISOLAT KB KOMPOS TERMOFILIK DESA BAYAT KLATEN MENGGUNAKAN FRAKSINASI AMONIUM SULFAT

 

Full Text PDF (216 kb)
Chem Info
Vol 1, No 1 (2013)
PEMURNIAN SELULASE DARI ISOLAT KB KOMPOS TERMOFILIK DESA BAYAT KLATEN MENGGUNAKAN FRAKSINASI AMONIUM SULFAT
Sinatari, Hanum Mustika ( Diponegoro University)
Article Info   ABSTRACT
Published date:
21 Jan 2013
 
Purification of cellulase enzymes derived from KB thermophilic composting isolates of Bayat Klaten using fractionation ammonium sulphate needs to be done so that cellulase can cuts cellulose chains more effectively. This study aims to gain cellulases from KB thermophilic composting isolates of Bayat Klaten is more pure than the crude cellulase extract. Purification of cellulases from KB isolates using fractionation ammonium sulphate with saturation 0-20% (F1), 20-40% (F2), 40-60% (F3), 60-80% (F4), and 80-100% (F5), followed by cellulase dialysis using cellophane membranes. Cellulases fractions activity was measured using Dinitrosalicylic acid (DNS) method and Lowry method. This study obtained cellulase with specific activity in the crude extract cellulase of 0.212 units/mg protein and the highest enzyme specific activity in fraction 2 (20-40% ammonium sulphate) of 0.749 U/mg protein. The specific activity of cellulase in fraction 2 increased 3,5 times from the specific activity of the crude cellulase extract.  
Copyrights © 2013