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Journal > Jurnal Mikrobiologi Indonesia > The Antimutagen Capacity of Synthesized Polyphenol Glycoside through Transglycosylation by CGTase Enzyme of Bacilluspolymyxa D4

 

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Jurnal Mikrobiologi Indonesia
Vol 6, No 1 (2001): JURNAL MIKROBIOLOGI INDONESIA
The Antimutagen Capacity of Synthesized Polyphenol Glycoside through Transglycosylation by CGTase Enzyme of Bacilluspolymyxa D4
Article Info   ABSTRACT
Published date:
30 Dec 2010
 
Polyphenol gycoside was synthesized through enzimatic trausglycosylation by cyclodeztrin glucanotransferaseI1.4-a-D-glucan 4-cz-D-1,4-glucano-transfcrssc or CGTase EC 2.4.1.19, of Bacillus poly,nyxa D4. Soluble starch andresorcinol were used as the aubstrat and the acceptor respectively. The transfer product was detected using thin layerchromatography as resorsinol glucoside. Purif.c*tion of transfer product was carried out using column chromatography and resorsinol glucoalde was collected In fraction of 20% methanol. The bioassay of mutagenesis was detected byformation of mutation induced by afiatoin B1 1 ig/ml In Salmonella sypl.ymurium TA9S. The effect of antimutagenesiswas evaluated using this culture on L-histidine deficiency medium containing resorcinol glucoside. Results showthat rcaorcinol glucoside like arbutin and resorcinol can inhibit niutagenesis at concentration of 25 mM.
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