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Journal > Jurnal Tumbuhan Obat Indonesia > ANTICANCER ACTIVITY OF ZINGIBER OFFICINALE AND PIPER RETROFRACTUM EXTRACT COMBINATION ON HELA CELL LINE

 

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Jurnal Tumbuhan Obat Indonesia
Vol 3, No 2 Des (2010)
ANTICANCER ACTIVITY OF ZINGIBER OFFICINALE AND PIPER RETROFRACTUM EXTRACT COMBINATION ON HELA CELL LINE
Ekowati, Heny ( Pharmacy Department, Medicine and Health Sciences Faculty, Jenderal Soedirman University)
Septiyaningsih, Septiyaningsih ( Pharmacy Department, Medicine and Health Sciences Faculty, Jenderal Soedirman University)
Harwoko, Harwoko ( Pharmacy Department, Medicine and Health Sciences Faculty, Jenderal Soedirman University)
Trisnowati, Trisnowati ( Pharmacy Department, Medicine and Health Sciences Faculty, Jenderal Soedirman University)
Article Info   ABSTRACT
Published date:
07 Feb 2013
 
The use of chemotherapy for cancer cause side effects since its affect to synthesis of nucleic acids and proteins both cancer cells and normal cells. The combination of plants used to enhance the anticancer activity and to min:imize side effects caused by anticancer drug. Red ginger rhizome (Zingtberofflcinale ev. Rubrum) and java pepper fruit (Piper retrofractum) are empirically used as an anti cancer. This study was conducted to investigate the cytotoxic and apoptotic activity of Zingiber officinale and Piper retrofractum extract and its effeet to p53 and caspase 9 expression on Hela cervical carcinoma cell. Cytotoxic assay was performed using MTT assay, apoptotic assay was performed by immunofluororescence method, using fluorochromes ethidium bromide and acridine orange. Expression of p53 and caspase 9 was examined by immunohistochemistry method. Cisplatin was used as positive control. The result showed that extract combination has cytotoxic activity on Hela cell line, with IC50 - 33.81 ug/ml, through apoptotic mechanism, increase expression of p53 tumor suppressor gene and caspase 9. Keywords: Zingiber officinale, Piper retrofractum, cytotoxic, Hela Cell Line
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