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Journal > Jurnal Tumbuhan Obat Indonesia > POTENSI CAMPURAN MINYAK ATSIRI RIMPANG TEMULAWAK DAN DAUN CENGKEH SEBAGAI INHIBITOR PLAK GIGI

 

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Jurnal Tumbuhan Obat Indonesia
Vol 3, No 2 Des (2010)
POTENSI CAMPURAN MINYAK ATSIRI RIMPANG TEMULAWAK DAN DAUN CENGKEH SEBAGAI INHIBITOR PLAK GIGI
Prasasti, Dwi ( Bagian Biologi Farmasi, Fakultas Farmasi Universitas Gadjah Mada)
Hertiani, Triana ( Bagian Biologi Farmasi, Fakultas Farmasi Universitas Gadjah Mada)
Article Info   ABSTRACT
Published date:
07 Feb 2013
 
Temulawak rhizome (Curcuma xanthorrlza) and cengkeh leaf CSyzygium aromaticum) essential oils have been reported to be potential inhibitors of planktonik growth and biofilm of S. mutans. This research aimed to reveal the potency of the essentialoils blend and the optimum compostition based on i15 inhibitory effect against planktonik growth and biofilm of S. mutans by an in vitro method. Blend composition of temulawak and cengkeh essential oils tested were 1:0; 0.75:0.25; 0.5:0. 1:0; 0.75:0.25; 0.5:0.5; 0.25:0.75 v/v; 0.25:0.75 v/v, respectively, in total concentration of 0.002%. The planktonic growth inhibitory assay was perfonned against S. mutans by micro dilution technique on nutrient broth media. Biofilm formation inhibition and degradation assay was condueted on BH!. Biofilm formation was indirectly assessed by staining with 1 % crystal violet. Data was obtained from plate reader in wavelength of 595 nm. The above assays were perfonned in triplicate. The results were used to detennine the SLD formula. Results showed that the essential oils blend did not significantly influence the planktonic growth inhibition but significantly increase the biofilm formation inhibitory percentage as well as degradation. Optimum composition was detennined as temulawak essential oils 55 and cengkeh 45. Keywords: essential oils bl end, temulawak rhizome, cengkeh leaf, S. mutans
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