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Journal > Jurnal ILMU DASAR > Production and Characterization of Enzyme β-Endoxylanase from Bacteria of Termite-intestinal System

 

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Jurnal ILMU DASAR
Vol 8, No 2 (2007)
Production and Characterization of Enzyme β-Endoxylanase from Bacteria of Termite-intestinal System
Article Info   ABSTRACT
Published date:
25 Jun 2013
 
A mesophile bacteria isolated from intestinal systems of local-soil termite, produced an extracellular   β-endoxylanase upon growth on xylan. Optimum production of the enzyme was found in media containing oatspeltxylanat37oC after sixth hours. The activities of its β-endoxylanase on oat-spelt xylan was investigated. Ithad an optimum pH and temperature, 5.0 and 40o C, respectively. However, pH stability occurred between 5.08.0.The enzyme was stable at 40o C for four hours and possessed a half life of four hours. β-endoxylanase had an apparent molecular mass of 45.000 to 66.200 Dalton as determined by SDS-PAGE. Analysis of zymogramusing SDS-Xylan-PAGE indicated that enzymes could degrade oat-spelt xylan as substrates. Keywords : intestinal-system bacteria,  production, characterization,β-endoxylanase
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